Abstract #T65

# T65
Effects of poor maternal nutrition during gestation on protein expression in the liver of lambs.
Katelyn K. McFadden*1, Maria L. Hoffman1, Kristen N. Peck1, Sarah A. Reed1, Steven A. Zinn1, Kristen E. Govoni1, 1Department of Animal Science, University of Connecticut, Storrs, CT.

Poor maternal nutrition during gestation can reduce growth and circulating growth factors secreted by the liver, as well as alter lipid metabolism; however, the mechanisms involved are not well understood. Previously, we reported that poor maternal nutrition altered liver expression of genes involved in lipid metabolism and the somatotropic axis in the offspring. Therefore, we hypothesized that poor maternal nutrition during gestation would alter expression of key proteins involved in lipid metabolism and the somatotropic axis in the liver of offspring. Multiparous ewes (n = 36) were individually housed and fed 100, 60, or 140% of NRC requirements beginning at d 31 ± 1.3 of gestation. Lambs were euthanized within 24 h of birth (1 d; n = 18) or at 3 mo of age (n = 15). Lambs from ewes fed 100, 60, or 140% will be referred to as CON, RES, and OVER, respectively. At euthanasia, whole livers were harvested, weighed and tissue samples collected. Protein was determined by Western immunoblot. Proteins were imaged using an Odyssey CLx Imaging System and quantified using Image Studio Lite program. Data were analyzed using PROC GLM. As previously reported, BW were 13% greater in OVER vs. CON at 1 d and 3 mo. Liver weight was 43% greater in OVER vs. CON at 1 d when adjusted for BW, but not at 3 mo. Based on previously determined changes in gene expression in the liver, sterol-regulatory element binding protein-I (SREBP-I), a regulatory gene of hepatic lipogenesis, IGF binding protein-4 (IGFBP-4) and IGFBP-3 were evaluated. At 3 mo, IGFBP-3 expression increased 16% in OVER vs. CON (P = 0.03) and IGFBP-4 tended to increase 88% in RES vs. CON (P = 0.08). Maternal diet did not affect protein expression of IGFBP-3 or −4 at 1 d or SREBP-I at either time point (P ≥ 0.11). The observed increase in IGFBP-3 protein expression in OVER parallels previously determined increases in gene expression and circulating concentrations of IGFBP-3. In conclusion, poor maternal nutrition alters gene, protein, and circulating concentrations of key growth factors, such as IGFBP-3, which may contribute to altered growth of offspring.

Key Words: IGF binding protein, liver, maternal nutrition