Solanine is a glycoalkaloid compound found in leaves, fruit and tubers of potato, tomato, and eggplant. Solanine poisoning was reported in various animal models such as mice, rabbit, and chicken. However, it is used as a treatment for human asthma. Some medicine used for treating human asthma has been used to induce myogenesis of bovine skeletal muscle. We hypothesized that α-solanine may affect myogenesis of bovine satellite cells isolated from different muscle depots. Bovine satellite cells were pronase-liberated from semimembranosus (SM) and longissimus dorsi (LD) muscle tissues of 3 newborn Hanwoo calves. Bovine SM and LD satellite cells were incubated with dulbecco modified eagle medium (DMEM) with 10% fetal bovine serum for proliferation and induced differentiation with DMEM with 3% horse serum. Bovine satellite cells were treated with various levels of α-solanine (control, 0.001, 0.01, 0.1, 1, and 10 μM). mRNA abundance for myosin heavy chain 1 (MHC1), MHC2X, glucose transporter 4 (GLUT4), myogenin, G-coupled protein receptor 43 (GPR43), and β2-adrenergic receptor (β2-AR) were measured by real-time quantitative PCR. Data were analyzed as a completely randomized design using the MIXED model, each treatment performed in triplicated. Means were considered different at P < 0.05. Relative MHC2X mRNA abundance was greater in both, SM and LD satellite cells with 1 μM solanine treatments compared with the control (P < 0.05). Relative level of MHC2X and GLUT4 were decreased in 10 μM solanine treatments (P < 0.05). Relative level of MHC2X and β2-AR were greater in SM satellite cell compared with LD satellite cells (P < 0.05). There was no tissue x dose interaction among MHC1 mRNA concentration (P > 0.05). These result indicated that α-solanine have a dose-dependent effect on MHC2X mRNA but did not affect to MHC1 mRNA in bovine satellite cell.