Traditionally removal of organic soils in a clean-in-place (CIP) cleaning cycle by alkaline, chlorinated detergents is carried out at temperatures ranging from 50 to 65°C. The objective of this research was to determine the cleaning efficiency of a newly formulated CIP solution called Cool CIP, a chlorinated alkaline cleaner developed by DeLaval Cleaning Solutions designed to function at low temperatures. Cool CIP is formulated to function at temperatures ranging from 32 to 40°C. The study was conducted over a 1-year period at the Michigan State University Dairy Plant. CIP cleaning cycles of a raw milk tanker truck and receiving lines, raw milk lines, and raw milk storage tanks were chosen to be included in the study. Effectiveness of Cool CIP was evaluated on each cleaning cycle by analysis of solution temperature, chlorine level, pre-wash and post-wash turbidity, Adenosine Tri-Phosphate (ATP) bioluminescence counts, as well as visual confirmation of overall cleanliness. Samples were collected at the beginning and end of the chemical recirculation step of each CIP cycle. ATP swabs were taken inside the vessel of each cycle, before and after each complete cleaning cycle. The results showed that Cool CIP was able to clean effectively at temperatures as low as 30°C as careful visual inspection of all systems passed after each cleaning cycle. The results also showed that all ATP tests were passable post-cycle on tests at 30°C. The raw line tanker truck receiving lines (n = 14) had 1011.6 ± 943.4 ATP units pre-cleaning, and 0 ± 0 ATP units post-CIP cleaning cycle. The raw milk lines (n = 7) had 10611.3 ± 25620.2 ATP units pre-cleaning, and 1.5 ± 4.8 ATP units post-CIP cleaning cycle. The raw milk tanks (n = 12) had 1381.1 ± 1116.2 ATP units pre-cleaning, and 0 ± 0 ATP units post-CIP cleaning cycle. This paper will present Cool CIP as an effective substitute for traditionally accepted high-temperature alkaline CIP cleaning regimens.