Heifers from southwest Louisiana were dry-lotted and evaluated for factors influencing estrus and conception. In October, spring born heifer calves (n = 121, 259 ± 150 kg) were received and processed with routine procedures before beginning a 120 d feeding trial. Heifers were visually assessed for temperament and percentage of Brahman influence. Temperament was evaluated as individual chute scores (CS) and denoted as 1–4 increasing with increased heifer excitability. Temperament was also evaluated as chute exit velocity (EV) using a rodeo event timer to calculate the speed of each animal exiting the squeeze chute. Brahman influence (BI) was determined by the phenotype and/or genotype of the heifers. Criteria for phenotype assessment were ear length and shape, hair type, coat color, and length of sheath. Individual BW and ADG were assessed monthly. Heifers were scanned by ultrasound at d 120 to determine RF, REA, and IMF. Intramuscular fat and RF decreased (P < 0.05) in heifers with greater BI. Cattle that were greater than 25% BI had lower (P < 0.05) ADG and final BW than did heifers with 0 to 25% BI. During 2 rounds of estrus detection, females were fitted with electronic estrus detection transmitters and monitored for estrus behavior. In round 1 (n = 121), BI did not make a difference (P > 0.1) in the duration of estrus or the estrous cycle length. Temperament, as assessed by CS and EV, did not influence (P > 0.1) duration of estrus or estrus cycle length. In the second round of estrus detection (n = 59), there was no relationship (P > 0.1) between conception and estrous cycle length, duration of estrus, RF, REA, ADG, or BW. Temperament and breed also did not affect (P > 0.1) conception rate. This aided in clarifying the relationships of weight gain, carcass characteristics, temperament and BI on estrus behavior and conception rates in heifers.