Abstract #27
Section: Animal Health
Session: Animal Health: Reproductive health & acute immune responses
Format: Oral
Day/Time: Monday 11:15 AM–11:30 AM
Location: Sebastian I-2
Session: Animal Health: Reproductive health & acute immune responses
Format: Oral
Day/Time: Monday 11:15 AM–11:30 AM
Location: Sebastian I-2
# 27
Preweaning plane of nutrition and Mannheimia haemolytica dose influence inflammatory responses to a combined bovine herpesvirus-1 and Mannheimia haemolytica challenge in postweaned Holstein calves.
K. P. Sharon*1,2, Y. L. Liang1, N. C. Burdick Sanchez2, J. A. Carroll2, P. R. Broadway2, M. A. Ballou1, 1Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX,, 2USDA-ARS, Livestock Issues Research Unit, Lubbock, TX.
Key Words: health, nutrition, respiratory
Preweaning plane of nutrition and Mannheimia haemolytica dose influence inflammatory responses to a combined bovine herpesvirus-1 and Mannheimia haemolytica challenge in postweaned Holstein calves.
K. P. Sharon*1,2, Y. L. Liang1, N. C. Burdick Sanchez2, J. A. Carroll2, P. R. Broadway2, M. A. Ballou1, 1Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX,, 2USDA-ARS, Livestock Issues Research Unit, Lubbock, TX.
To determine whether previous plane of milk replacer nutrition (PON) and M. haemolytica (MH) dose influences inflammatory responses to a combined viral-bacterial respiratory challenge, Holstein calves (1d of age; n = 30) were assigned to treatments in a 2 × 3 factorial with preweaned PON and dose of MH as main effects (n = 5/treatment). Calves were fed either a low (LPN; n = 15) or a high PON (HPN; n = 15) from birth through weaning. Calves fed the LPN were fed 445 g DM/d of milk replacer until weaning, and HPN calves were fed 830g DM/d of milk replacer from 1 to 10d and 1080g from 11d until weaning. Calf starter and water were offered ad libitum. Calves were step-down weaned beginning at 54d and moved into an enclosed barn at 70d. Indwelling rectal temperature (RT) devices and jugular catheters were inserted at 80d. Calves were challenged with 1.5 × 108 PFU/mL/nostril of bovine herpesvirus-1 (BHV-1) at 81d and with 106, 107, or 108 cfu of MH at 84d. Blood samples were collected at varying intervals respective to BHV-1 and MH challenges. There was a PON × MH interaction (P < 0.01) on RT, where calves receiving 108 MH had the greatest increase among HPN calves, but the least among LPN calves. Haptoglobin (Hp) concentrations were greater (P = 0.04) in LPN vs HPN calves after BHV-1 challenge. There was a time x MH interaction (P < 0.01) for Hp concentrations after the MH challenge, where calves receiving 107 MH had the greatest Hp concentrations at 24 h, and calves receiving 108 MH the greatest at 144h. During the BHV-1 challenge, neutrophil concentrations (PMN) and change in total leukocytes were greater (P < 0.01) among LPN vs HPN calves. After the MH challenge PMN were greatest (P ≤ 0.05) in calves receiving 108 MH at 5 and 24h. During the MH challenge calves receiving 108 MH had the greatest (P < 0.05) IL-6 and TNFα concentrations. These data demonstrate that LPN calves responded more severely to a combined viral-bacterial respiratory challenge and greater doses of MH increase the acute inflammatory response and prolong inflammation.
Key Words: health, nutrition, respiratory
