Cecal and ileal cannulations have been utilized to determine equine digestibility and gastrointestinal physiology, but these cannulations were limited to a single segment of the digestive tract. While these earlier cannulations were successful, the recovery process was difficult for the single cannulation; and thus, the objectives of this case study were to document the post-operative recovery of a dual cannulation surgery in equines and to determine the effectiveness of establishing a dual cannulated research herd. Eight Hackney/Shetland pony cross mares (BW: 200 to 250 kg) were selected for development of a cannulated herd. Initial incisions for placement of the ileal and cecal cannulas were done on the same day. A novel ileal cannula was constructed of a biologically compliant silicone, which included a flange to assist with surgical anchoring to improve stoma formation. For the cecal cannula, a rumen cannula (#7C; Bar Diamond, Parma, ID) was placed into the cecal fistula similar to previous studies. Ileal stomas adhered after 14 d and the cecal stoma at 10 to 14 d post-surgery. Full vital analysis occurred every 4 h for 4 wk post-surgery. Due to granulation at the cecal site, the most common complication for all ponies was increased rectal temperature of 38.9 to 40.9°C from 4 to 10 d post-surgery with a mean ranging from 37.8 to 38.5°C and a standard deviation ranging 0.59 to 1.4. Five ponies showed signs of colic approximately 1 to 2 times during recovery. After surgical recovery, 5 ponies remained healthy during 7 mo of observations, demonstrating vital signs within normal ranges and no colic signs. The 3 ponies that died had intestinal leakage into the abdominal cavity due to lack of adherence of ileum to the body wall resulting in peritonitis within 2 wk post-surgery. Compared with other tubing or polyvinyl chloride options, the current novel ileal cannula design was effective as the ileal cannula design formed a stoma and was easily maintained for the remaining 5 ponies. With a survival rate of 63% at 7 mo post-surgery, the dual cannulation of the ileum and cecum was considered successful.