Low-fat corn DDGS (L-DDGS) is available for pig feeding. However, information is lacking on fermentation characteristics of L-DDGS in the pig intestine and on the effect of fiber-grading enzymes (carbohydrases) on fermentation characteristics of L-DDGS. Thus, we determined porcine in vitro digestion and fermentation characteristics of regular (R-DDGS) and L-DDGS without or with a carbohydrase that supplied 4,880 units of xylanase and 608 units of β-glucanase per treated feedstuff in a completely randomized design. Samples were hydrolyzed in 2 steps using pepsin and pancreatin. Subsequently, residues were incubated in a buffer solution with minerals and fresh pig feces as inoculum. Gas production was measured for 72 h, and modeled to estimate kinetics of gas production. Concentration of VFA per unit weight of residue incubated or feedstuff was measured in fermented solutions. Carbohydrase did not affect in vitro digestibility of DM (IVDDM) and total gas production. However, IVDDM for L-DDGS (61.1%) was greater (P < 0.05) than that for R-DDGS (54.7%). Also, total gas production for L-DDGS (129.9 mL/g DM) was greater (P < 0.05) than that for R-DDGS (119.0 mL/g DM). Total VFA production per unit weight of residue incubated for L-DDGS (2.66 mmol/g DM) was lower (P < 0.05) than for R-DDGS (2.93 mmol/g DM). Also, total VFA production per unit weight of feedstuff for L-DDGS (1.19 mmol/g DM) was lower (P < 0.05) than for R-DDGS (1.51 mmol/g DM). Carbohydrase and feedstuff interacted (P < 0.05) on total VFA production per unit weight of feedstuff such that the carbohydrase tended to increase (P = 0.09) total VFA production per unit weight of feedstuff for R-DDGS, but did not affect total VFA production per unit weight of feedstuff for R-DDGS. In conclusion, L-DDGS may be more digestible in the small intestine of pigs than the R-DDGS, whereas R-DDGS may be more fermentable in hindgut of pigs than the L-DDGS. The carbohydrase used in the present study may have limited effect on porcine small intestine digestibility and hindgut fermentation of R-DDGS and L-DDGS.