Abstract #W66
Section: Breeding and Genetics
Session: Breeding and Genetics: Genomic methods and application - Beef
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
Session: Breeding and Genetics: Genomic methods and application - Beef
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
# W66
GWAS between single nucleotide polymorphisms with beef fatty acid profile in Nellore cattle using the single-step procedure.
Marcos V. A. Lemos*1, Hermenegildo L. J. Chiaia1, Mariana P. Berton1, Fabiele L. B. Feitosa1, Carolyn Aboujaoude1, Adrielle M. Ferrinho2, Lenise F. Mueller2, Joyce J. M. Furlan2, Angelica S. C. Pereira2, Lucia G. Albuquerque1, Fernando Baldi1, 1State University of São Paulo, Jaboticabal, São Paulo, Brazil, 2University of São Paulo, Pirassununga, São Paulo, Brazil.
Key Words: Bos indicus, fatty acid composition, genetic markers
GWAS between single nucleotide polymorphisms with beef fatty acid profile in Nellore cattle using the single-step procedure.
Marcos V. A. Lemos*1, Hermenegildo L. J. Chiaia1, Mariana P. Berton1, Fabiele L. B. Feitosa1, Carolyn Aboujaoude1, Adrielle M. Ferrinho2, Lenise F. Mueller2, Joyce J. M. Furlan2, Angelica S. C. Pereira2, Lucia G. Albuquerque1, Fernando Baldi1, 1State University of São Paulo, Jaboticabal, São Paulo, Brazil, 2University of São Paulo, Pirassununga, São Paulo, Brazil.
The aim of this study was determine genomic regions associated with the profile of beef fatty acid (FA) of Nellore cattle finished in feedlot using the single-step method. A total of 1,616 genotypes and 963 phenotypes were used. The FA profile was analyzed in Longissimus thoracis samples using a gas chromatography, with a 100-m capillary column. The following fatty acids were analyzed: lauric (C12:0), palmitic (C16:0), stearic (C18:0), oleic (C18:1 cis-9), linoleic (C18:2 cis-6), CLA (C18:2 cis-9 trans-11), CLA (C18:2 trans10 cis12), linolenic (C18:3 n3), myristic (C14:0), myristoleic (C14:1), docosahexaenoic (C22:6 n3), elaidic (C18:1 n9t), vaccenic (C18:1 t11), arachidonic (C20:4 n-6) eicosatrienoic (C20:3 n6 cis-8,11,14) and alfa-linolenic (C18:3 n6). The animals were genotyped with the BovineSNP BeadChip (High-Density Bovine BeadChip). After quality control of genotypes, a total of 470,000 SNPs and 1,556 samples remained. The model used for the (co)variance and genetic parameter estimation included the random genetic additive direct effect, the fixed effect of the contemporary groups, and the animal’s slaughter age as a covariable. To determine the areas of QTL, segments that were ≥ 1% of the additive genetic variance were chosen. For identification and positioning of these segments, the database available in the “National Center for Biotechnology Information” and Ensembl Genome Browser were used. A total of 115 genomic regions (1-Mb SNP windows) associated with the FA profile were identified many of these regions were previously detected in other cattle breeds, like the gene ELOVL5 (fatty acid elongase 5) associated with the C20:4 n-6 FA, the ESRRG (estrogen receptor-related gamma gene) was associated with the C12:0 FA and the PCYT1A, TCTEX1D and GALNTL6 were associated with C18:2 cis-9 cis-12 n-6, C14:0 and C16:0 FA. The genes present in these regions may help to explain the genetic basis of FA profile in Bos indicus cattle, contributing to better selection of these traits associated with improvement of human health.
Key Words: Bos indicus, fatty acid composition, genetic markers
