Abstract #M133
Section: Graduate Student Competition
Session: ADSA Production Division Graduate Student Poster Competition, MS
Format: Poster
Day/Time: Monday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
Session: ADSA Production Division Graduate Student Poster Competition, MS
Format: Poster
Day/Time: Monday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
# M133
The effect of estrus expression on target genes in the endometrium, conceptus and corpus luteum of cows.
Saeideh Davoodi*1, Reinaldo F. Cooke2, Arthur C. C. Fernandes1, Bruno I. Cappellozza2, Jose L. M. Vasconcelos3, Ronaldo L. A. Cerri1, 1Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada, 2Eastern Oregon Agricultural Research Center, Oregon State University, Burns, OR, 3Faculdade de Medicina Veterinária e Zootecnia, UNESP – Botucatu, SP, Brazil.
Key Words: conceptus, corpus luteum, endometrium
The effect of estrus expression on target genes in the endometrium, conceptus and corpus luteum of cows.
Saeideh Davoodi*1, Reinaldo F. Cooke2, Arthur C. C. Fernandes1, Bruno I. Cappellozza2, Jose L. M. Vasconcelos3, Ronaldo L. A. Cerri1, 1Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, Canada, 2Eastern Oregon Agricultural Research Center, Oregon State University, Burns, OR, 3Faculdade de Medicina Veterinária e Zootecnia, UNESP – Botucatu, SP, Brazil.
The aim of this study was to test the effect of estrus expression at artificial insemination (AI) on the endometrium, conceptus and corpus luteum (CL) gene expression. Twenty-three multiparous nonlactating Nelore cows were enrolled on a estradiol and progesterone (P4) based timed-AI protocol (AI = d 0), then slaughtered for endometrium, CL and conceptus collection on d 19. Body condition score (BCS), blood samples for analysis of P4 and ultrasound examination was performed on d 0, 7 and 18 of the experiment. RNA extraction and qRT-PCR analysis of 58 target genes were analyzed on tissues. Data were checked for normality and analyzed by ANOVA for repeated measures using proc GLM, MIXED and UNIVARIATE. Estrous expression had no correlation with parameters such as BCS, pre-ovulatory follicle and CL diameter, P4 concentration in plasma on d 7 and 18 after AI and IFN-tau concentration in the uterine flushing (P > 0.05); however, a significant increase was observed in conceptus size (P = 0.02; 38.3 ± 2.8 vs. 28.2 ± 2.9). The majority of transcripts affected by estrous expression in the endometrium belong to the immune system and adhesion molecule family (MX1, MX2, MYL12A, MMP19, CXCL10, IGLL10 and SLPI; P ≤ 0.05). Genes related to apoptosis, P4 synthesis and prostaglandin receptor were downregulated (CYP11A, BAX and PGR; P < 0.05) in the CL tissue of cows in estrus. In addition, 4 genes were identified as differentially expressed in the 19 old conceptus from cows observed in estrus (ISG15, PLAU, BMP15 and EEF1A1; P < 0.05). There was an interaction (P < 0.05) between estrus expression and P4 concentration on d 7 which mainly affected genes involved in immune system (MX1, MX2, TRD, SLPI and IGLL1). This study demonstrated that estrous expression at the time of AI, in spite of ovulation being induced by estradiol, can alter the gene expression profile in reproductive tissues during the preimplantation phase. These effects seem to be more pronounced in endometrium during the time of dynamic remodeling to prepare a receptive uterus for embryo implantation.
Key Words: conceptus, corpus luteum, endometrium
