Development of oral vaccine is necessary in animal husbandry field because of not only its convenience in treatment but also its capability inducing mucosal immune response. M cells are well-known as antigen collecting portals for GALT (gut associated lymphoid tissue) in intestinal tract, thus targeted delivery of vaccine molecules to the M cells could be a promising strategy to improve efficiency of oral vaccine. Previously, our research group have identified an M cell targeting peptide moiety, CKS9, by phage display technique using in vitro M cell model consisting of coculture system with Caco-2 (human colon carcinoma cells) and human Raji B cells. In this study, we constructed a recombinant lactic acid bacteria, Lactobacillus plantarum producing a model antigen, M-BmpB, the BmpB (surface membrane protein originated from Brachyspira hyodysenteriae) conjugated with CKS9, to validate its potency as an efficient animal oral vaccine. We ascertained that the fusion protein, M-BmpB, was expressed as soluble form in the cytoplasm of L. plantarum by SDS PAGE and Western blot and confirmed its M cell targeting property in contrast to original BmpB (without CKS9) and P-BmpB (with unrelated peptide ligand) by in vivo closed ileal loop assay. In in vivo immunization assay (Balb/C, n = 5 in each group), Oral administration of L. plantarum producing M-BmpB (LP25-M-BmpB) to mice revealed significant improvement in induction of both serum IgG (P < 0.05) and fecal IgA (P < 0.01) against BmpB compared with control groups. Our results suggest that the recombinant lactic acid bacteria, such as L. plantarum, producing certain pathogenic antigen with M cell targeting strategy could have a great potential to develop an effective and convenient oral animal vaccine system.