Hypocalcemia is a periparturient metabolic disorder that is costly to the US dairy industry. One strategy to reduce incidences is to feed anionic supplements during the close-up period. The effectiveness of this strategy may be monitored by evaluating urine pH of cows, with an effective preventative pH in the range of 6.0 to 7.0. There are multiple commercial urine pH strips available to test the acidity of urine. The objective of this study was to evaluate several pH test strips for accuracy in determining pH of cow urine. Urine was collected from 4 mid-lactation Holstein dairy cows, composited, and distributed into ten 250-mL samples. The pH of each sample was then adjusted, using 12 N HCl and a laboratory pH meter, to 1 of 10 pH values: 5.5, 6.0, 6.25, 6.5, 6.75, 7.0, 7.25, 7.50, 7.75, and 8.0. The pH of each standard was recorded at the end of sampling and the average of the 2 values (5.68, 6.10, 6.33, 6.63, 6.81, 7.06, 7.33. 7.57, 7.76, and 8) was used to compare the accuracy of the strips. Seven evaluators assessed each pH sample with 9 commercially available pH strips to determine equivalence between the pH strip reading and the pH of the prepared standard. Confidence intervals (90%) were calculated for each pH strip and sample combination and compared with the standard. Seven of the 9 strips fell within the 90% confidence interval ranges for 1 to 3 urine samples and 1 strip was within the range for 5 samples. Of the pH strips tested, only 1 was able to identify a correct pH for 7 of the 10 urine samples. Based on our results, this strip could be used to identify urine pH in a range from 5.7 to 7.75. These results depict the variation in the accuracy of the test strips to detect urine pH. Producers must be aware of these variations as they utilize urine pH to measure the effect of feeding anionic supplements in a transition cow program.