This study was conducted to evaluate the effects of reducing dietary cation-anion difference (DCAD; Na⁺K-Cl⁻S, mmol/kg DM) with vitamin D (VD) supplementation on plasma levels of Ca and its modulators, and ration palatability for goats. Eighteen healthy female goats were randomly allocated to 3 treatments with 6 goats per treatment and were fed 3 DCAD levels at 150, −150, −150 for control, treatment 1, and treatment 2, respectively. Anionic salts were included to reduce DCAD level for treatment 1, and VD was added in treatment 1 for treatment 2. Urine pH; plasma Ca, P, PTH, CT, 1,25-(OH)₂D₃, vitamin D receptor (VDR), Ca-binding protein (CaBP-D9k) concentrations; and dry matter intake (DMI) were measured in the trial. Urine pH was reduced (P < 0.05) as DCAD decreased for treatment 1 (6.71) and treatment 2 (6.67) over control (7.63). Compared with control, plasma Ca was increased for treatment 1 (P > 0.05) and treatment 2 (P < 0.05). There was no difference in plasma P and PTH for control, treatment 1, and treatment 2 (P > 0.05). Treatment 2 had increased plasma 1,25-(OH)₂D₃ relative to control and treatment 1 (P < 0.05) and tended to reduce plasma CT over treatment 1 (P < 0.1). Moreover, reducing DCAD plus VD for treatment 2 resulted in higher plasma VDR and CaBP-D9k than that of control (P < 0.05). Levels of DMI were unaffected among 3 treatments (P > 0.05). These results suggested that plasma Ca homeostasis could be more available to maintain by reducing DCAD plus VD in the present study.