The aim of this study was to verify association among skin thickness (ST) and resistance to the Rhipicephalus microplus ticks on Simmental animals, artificially infested with ticks. Eighteen Simmental heifers, with about to 450 age days and belonging a farm in the Avaré/SP/Brazil were used. ST was measure on the left side behind the shoulder of animal by a tuberculin caliper. Three artificial infestations were performed using approximately 20,000 R. microplus larvae on each animal. Counts female ticks were measured in the 19th and 23th range days after infestations, what only left side of the animal and engorged females with sizes larger than 4.5 mm were considered. Total tick counts (TTC) and payback percentages (PP) variables were transformed, respectively, in log₁₀ (n + 1) and PP^0.25. MIXED procedure of SAS considering repeated measurements was used. Models considering the skin thickness group (STG), divided into thin leather and thick leather, infestation (IN) and their interactions as fixed effects were used in the analyzes for both variables. Mean comparisons were performed using Tukey-Kramer test (P < 0.05). Repeatability coefficient (R) of the variables was estimated by intraclass correlation between the same animal measures. The IN effect was significant (P < 0.05) for the studied variables. The STG mean were 7.11 ± 0.74 and 11.24 ± 0.86 for thin leather and thick leather, respectively. Means values observed, in chronological order, were 0.33 ± 0.29, 1.98 ± 0.22 and 2.36 ± 0.38 to TTC and 1.61 ± 1.35, 6.64 ± 0.83 and 8.40 ± 1.64 for PP. It is probably that the amount of detached female of animals significantly increased the infesting larval on pastures, caused significantly increased (P < 0.05) of TTC and PP in the following counts. Value of R were 0.61 for both variables, suggesting that resistance to ticks measures observed in the same animal have similar values in artificial infestations data. There was no significant effect of STG, indicating that the ST trait is not an attribute that influences in cattle resistance to ticks.