Abstract #W112
Section: Comparative Gut Physiology
Session: Comparative Gut Physiology
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
Session: Comparative Gut Physiology
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Gatlin Ballroom
# W112
Effect of a post-weanling diet supplemented with gut health-enhancing feed additives on ileum transcriptome activity and serum cytokines in piglets challenged with lipopolysaccharides (LPS).
Nathalie Bissonnette*1, Xian-Ren Jiang1,4, Jacques J. Matte1, Guylaine Talbot1, Frédéric Guay2, Joshua Gong3, Qi Wang3, Valentino Bontempo4, Martin Lessard1, 1Dairy & Swine Research and Development Centre Agriculture and Agri-Food Canada, Sherbrooke, Quebec, Canada, 2Département des Sciences Animales, Université Laval, Québec, Québec, Canada, 3Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Ontario, Canada, 4Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
Key Words: weaned swine feeding, gut health-enhancing feed additive, intestinal disease
Effect of a post-weanling diet supplemented with gut health-enhancing feed additives on ileum transcriptome activity and serum cytokines in piglets challenged with lipopolysaccharides (LPS).
Nathalie Bissonnette*1, Xian-Ren Jiang1,4, Jacques J. Matte1, Guylaine Talbot1, Frédéric Guay2, Joshua Gong3, Qi Wang3, Valentino Bontempo4, Martin Lessard1, 1Dairy & Swine Research and Development Centre Agriculture and Agri-Food Canada, Sherbrooke, Quebec, Canada, 2Département des Sciences Animales, Université Laval, Québec, Québec, Canada, 3Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, Ontario, Canada, 4Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
This study aimed to evaluate the impact of dietary feed additives with supplemental vitamins and trace minerals (DS) on the ileum and on systemic immune responses to a LPS challenge in low- (LW) and high-weight (HW) weaned piglets. At 20 ± 1 d of age (weaning), litters (12 piglet each) from 32 sows were randomly assigned to 4 dietary groups: control diet (CTL), CTL diet + chlortetracycline (ATB) or + DS, or DS + bovine colostrum instead of spray-dried animal plasma (DS-BC). Within each litter, 2 LW, 2 medium-weight (MW) and 2 HW piglets were identified. At 37 d, LW and HW piglets received intra-peritoneal LPS injection (200 µg). Piglets were bled before the injection (T0) and at slaughter, 4 h (T4; 1 LW and 1 HW) and 18 h (T18; 1 LW and 1 HW) post-injection. Microarray analysis was performed on LW, MW, and HW ileal tissues. The dietary feed treatments had no effect on the basal transcriptome level of the in MW piglets or on blood concentrations at T0 of tumor necrosis factor-α (TNF-α), IL-8, and IL-10. At T4, LW piglets secreted more TNF-α (P = 0.05) and more (tendency) IL-8 (P = 0.11) and IL-10 (P = 0.10) than HW piglets. LPS altered the expression of 3,078 ileal genes with the false discovery rate (FDR) at 0.05. There was no diet × LPS interaction on ileal gene expression on T4 vs T18 vs unchallenged MW piglets. However, a diet effect was observed on gene expression for LW piglets using fold change > 1.5 for the T4/MW ratio. Confirmed by QPCR, T4/MW in DS+BC ileal tissue of the apoptotic CD163 gene was lower than in ATB’s for LW piglets (P = 0.02), whereas the corresponding effect in HW was a tendency (P = 0.07). The T4/MW ratio of the acute phase (SAA2 and S100A12), SOD2, and heat shock protein (DNAJB1 and HSP90AA1) genes was also lower in DS than ATB (P < 0.05) for LW piglets. The chemokine (CXCL9) T4/MW ratio was lower in both DS and DS+BC than ATB’s (P < 0.02) for LW and HW piglets. In conclusion, LW piglets developed a more pronounced inflammatory response than HW piglets and dietary feed additives supplemented with micronutrients attenuated the ileal gene response to LPS challenge.
Key Words: weaned swine feeding, gut health-enhancing feed additive, intestinal disease
