Bio-stimulation is the stimulus evoked by the presence of a male which induces sexual and oestrus expressions in females through genital stimulation, pheromones, and or other external cues. The aim of this study was to comparatively evaluate the effects of 2 bio-stimulation methods on lifetime reproductive performance of sows, arranged in a 2x3 factorial experiment. Thirty sows were assigned per group to each of 2 bio-stimulation protocols, twice daily (08:30 to 09:00 and 16:00 to 16:30) per 30 min duration in a 4-week observation per parity over 3 parities. The bio-stimulation protocols involved (1) fence-line boar exposure (FBE): in which sows were exposed to fence-line boars during oestrus detection and (2) physical boar exposure (PBE): in which sows received physical contact with the boar during oestrus detection. Following the bio-stimulation applications to each sow treatment group, oestrus was synchronised in sows by a single subcutaneous injection of P.G. 600 (400 IU PMSG with 200 IU HCG/5 mL dose/animal; Intervet Inc., Millsboro, DE). After the onset of oestrus, sows on each treatment were artificially inseminated using semen from the same boars and collections. All experimental females received inseminations of 3.5 × 10⁹ sperm/80 mL at 24 h after onset of oestrus. Physical boar exposure before oestrus induction (PG600) evoked the highest (P ≤ 0.01) reproductive performance in sows in parity 3 compared with those in parity 2 and 1, respectively. Sows that received physical boar exposure in parity 3 produced a higher (P ≤ 0.05) oestrus expressions (98 ± 23.1 vs. 77.5 ± 25.5%), Non-return rate (92.1 ± 6.3 vs. 74.4 ± 3.5%), farrowing rate (88.2 ± 16.5 vs. 63.8 ± 7.1%,), litter size (12 ± 0.02 vs. 8 ± 0.05) and live piglets (10 ± 0.05 vs. 6.5 ± 0.02), respectively compared with those from sows that received fence-line boar exposure in parity 3. Results suggest that direct exposure of boars to sows before artificial insemination following oestrus induction progressively enhances the lifetime reproductive performance in South African Mukota sows.