The objective of this experiment was to evaluate the effect of the dietary inclusion of calcium lactate (CL), calcium bio lactate (CBL) or calcium carbonate (CC) at NRC calcium level, in the presence of 0, 3%, 6% or 9% beef tallow on improving the performance, intestinal digestion and absorption of broiler chicks. Where, CBL derived from reacted between calcium hydroxide and biologically lactic acid secreted from lactic acid bacteria based on probiotic. Three hundred and six 1-d-old Cobb 500 broiler chicks were fed 12 treatments containing 3 calcium sources and 4 fat levels in a 3 × 4 factorial design with 3 replicates, 10 chicks each. Weight gain (WG), feed intake (FI), feed conversion rate (FCR), blood composition, nutrient digestibility and soap formation were recorded up to 7 weeks of age. The weight gain of broilers fed diets containing CBL with 6% tallow fortified diet was higher than those fed CL plus 6% tallow. Improvement (P < 0.05) in FCR was observed when broilers fed diets contained CL or CBL compared with those fed diets contained CC with different fat levels. Abdominal fat was increased (P < 0.05) when broilers fed diets containing CBL with 9% fat than those fed the other calcium sources and fat levels. The digestibilities of all nutrients were higher (P < 0.05) with broilers fed diets contained CBL or CL plus 6% tallow than those fed diets contained CC with different fat levels. The highest amount of excreted fat as soap formation were noticed for broilers fed diets contained CC with 9% tallow, while the lowest values observed with those fed diets contained CBL with 6% or 9% tallow. Total blood lipid of broilers fed diets contained CL or CBL with 6 or 9% tallow were greater (P < 0.05) compared with those fed the other treatments. Broilers fed diets contained CBL recorded the highest nitrogen and calcium retained compared with those fed diets contained CL or CC. It is concluded that the calcium lactate or calcium bio lactate based on probiotic were effective improving fat absorption and calcium retention with positive effect on broiler performance.