Abstract #26

# 26
Bovine viral diarrhea diagnostic testing results in the Intermountain West—Comparison between test methods, age, sex and beef versus dairy breeds.
David J. Wilson*1, Thomas J. Baldwin1, E. Jane Kelly1, Arnaud VanWettere1, Gordon Hullinger1, 1Utah State University, Logan, UT.

Bovine viral diarrhea (BVD) is an important cause of respiratory, gastrointestinal, and reproductive disease in cattle. The objectives were to calculate prevalence of BVD (“detected” test results) among all bovine samples tested for BVD at the Utah Veterinary Diagnostic Laboratory from 2009 to 2013, and to compare results by sex, age, dairy vs. beef breeds (if provided) of the cattle, and BVD test methods. For necropsied animals, most were tested by Ag capture ELISA, but fetuses were usually tested by PCR. Cattle were mainly from Utah, but also from surrounding states in the Intermountain West of the United States. This was not a planned experiment, but Chi-squared was used to test for significant differences in BVD prevalence between age, sex, breed and test methods. BVD was detected in 105/8,975 samples (1.2%), including 22/180 necropsies (12.2%). Test methods and results are given in Table 1. There were no significant differences in BVD detection by age or sex. Dairy breeds (P = 0.07), all necropsied animals, and those tested with PCR were significantly more likely to test as “detected” with BVD. The overall prevalence of >1% and the 8% prevalence in aborted fetuses demonstrates that despite the low reported prevalence of persistently infected cattle, BVD remains an important bovine disease. Table 1. Results by BVD test method, by age, sex, or breed, and by dairy versus beef breeds
ItemTested, no.BVD detected, no. (%)
BVD test method
 Ag Capture ELISA7,69279 (1.0)
 Serum ELISA1,19519 (1.6)
 PCR887 (8.0)*
 Necropsy (all methods)118022 (12.2)*
Age, sex or breed
 Male2155 (2.3)2
 Female3829 (2.4)2
 Fetus363 (8.3)2,3
 Immature1834 (2.2)2 4
 Adult754 (5.3)2 5
 Dairy675425 (3.3)
 Beef71,60026 (1.6)
1Subset of above 3 categories. 2NSD among sex, age, or by test method within sex, age or breed. 3All 3 by PCR at necropsy. 4Three by serum ELISA, 1 by Ag Capture ELISA, of 6 necropsies (16.7%). 5Two by serum ELISA, 2 by Ag Capture ELISA, of 24 necropsies (8.3%). 695.0% Holsteins, 3.8% Jerseys. 760.0% Angus, 22.8% “Beef.” Higher prevalence of “BVD detected” results, P < 0.0001; †P = 0.07*

Key Words: BVD, bovine, prevalence