This study was conducted to evaluate effects of dietary supplementation of nucleotide (disodium 5 guanylate and disodium 5 inosinate) on the growth performance and plasmatic lipid profile of weaned piglets. Eighty-four weaned piglets of 21 d of age (6.04 ± 0.25 kg average initial BW) were allotted to 4 dietary treatments with 7 pens per treatment and 3 pigs per pen, in a randomized complete block design. The treatments were dietary supplementation with 0% (control); 0.2%; 0.4% or 0.6% nucleotide of diet during 34 d. Performance was determined from 21 to 36, 21 to 48 and 21 to 55 d of age. Blood samples were collected to determinate lipid profile (triglycerides, total cholesterol, HDL cholesterol and LDL+VLDL cholesterol) on 21 and 55 d of age. Data were submitted to ANOVA using PROC GLM of SAS and the effects of supplementation levels of nucleotide were studied through regression analysis. During the first period (21 to 36 d of age) no differences were detected on the performance. During the second period (21 to 48 d of age) a quadratic effect (P < 0.01) of the levels of nucleotide was verified on average daily feed intake and average daily weight gain, with optimal response to the levels of 0.31% and 0.32%, respectively . During the whole experimental period (21 to 55 d of age) there was a quadratic effect of the levels of nucleotide (P < 0.01) on average daily weight gain with optimal response to the level of 0.31%. There was no significant effect on triglycerides, total cholesterol, HDL cholesterol and LDL+VLDL cholesterol. The overall results suggest that dietary nucleotide supplementation at 0.32% improves the performance of weaned piglets.