Abstract #843

# 843
Vitality and morphology of Boer buck spermatozoa stored fresh for 72 hours.
Olumide A. Ajao*1, Daniel M. Barry1, Kow K. Benyi1, 1University of Venda, Thohoyandou, Limpopo Province, South Africa.

The study was aimed at evaluating the effects Biladyl and Triladyl extenders on the percentage live proportion and morphological status of the Boer buck spermatozoa when stored at 5°C, 12°C and 17°C in programmable refrigerators for 72 h. Four (n = 4) healthy Boer bucks aged 3.12 ± 0.55 years were ejaculated using an artificial vagina (AV) once every 4 d for 6 replicates. Semen was extended at ratio 1: 5 v/v (semen to extender). All smears were duplicated. Semen samples were evaluated for percentage live spermatozoa and morphological status after every 12 h in 72 h of storage. Data were analyzed by ANOVA using the GLM procedure of Minitab. Spermatozoa viability and morphological correlations between extender type and temperature were assessed using the principal component analysis (PCA). The interaction between extender type and storage temperature indicated no significant difference (P > 0.05) on the proportion of live spermatozoa. The percentage live spermatozoa in Biladyl stored at 12°C was 76.6% compared with the higher percentage of live spermatozoa in Triladyl (80.0%) which were kept at 12°C. Among the conditions storage tested, temperature 12°C kept the least number of spermatozoa alive after the 72 h of storage with 69% of live spermatozoa. The percentage of live spermatozoa found in Triladyl after 72 h at 17°C was highest (86.3%). Spermatozoa kept in Biladyl at 12°C and in Triladyl at 12°C statistically produced higher morphological abnormality with (P < 0.05) effect than the morphological abnormality discovered in Biladyl 17°C and Triladyl 17°C. Temperature 12°C had the highest percentage of morphologically abnormal spermatozoa (23.33%) in Biladyl, followed by the Triladyl extender (22.46%) stored at 12°C, followed by the percentage found in Biladyl stored at 12°C (18.68%) and lastly by the 17.46% of sperm found in semen extended with Triladyl stored at 17°C. With appropriate protocol, Biladyl and Triladyl extenders can keep acceptable percentage of goat spermatozoa alive when stored at 5°C, 12°C and 17°C up to 72 h.

Key Words: extender, vitality, morphology