Abstract #629

# 629
Motility of Boer buck spermatozoa stored fresh for 72 hours.
Olumide A. Ajao*1, Daniel M. Barry1, Kow K. Benyi1, 1University of Venda, Thohoyandou, Limpopo Province, South Africa.

This study was aimed at evaluating the effects of Biladyl and Triladyl extenders, ambient and refrigerated temperatures (5°C, 12°C and 17°C) on the motility of Boer buck spermatozoa stored for 72 h period of storage. Four (n = 4) healthy Boer bucks aged 3.12 ± 0.55 years were ejaculated using artificial vagina (AV) once every 4 d for 6 replicates. The semen samples were pooled and divided into 2 equal halves. One half was extended in a 2-step Biladyl extender (semen sample plus Biladyl Fraction A made up half of the final volume) and the other with Triladyl, both at ratio 1: 5 v/v (semen to extender). Each of the extended semen divisions was further divided into 4 equal parts; one of the 4 was stored at ambient temperature and the others at 5°C, 12°C and 17°C. Data were analyzed by ANOVA for a 2 × 4 x 6 factorial in a completely randomized design, using the GLM procedure of Minitab (Minitab 2013). Spermatozoa motility was evaluated using Sperm class Analyzer (SCA) after every 12 h for 72 h. Extender type (E) and storage temperature (T) had highly significant effects (P < 0.01) on the total motility (TM) of Boer buck spermatozoa kept at the refrigerated temperatures after 72 h of storage compared with the TM of spermatozoa stored at ambient temperature which were the lowest in both extenders. Biladyl had 68% TM on spermatozoa kept at ambient temperature, 85% TM at 5°C, 89% TM at 12°C and 90% TM at 17°C; and Triladyl had 64% TM at ambient temperature, 88% TM at 5°C, 89% TM at 12°C and 90% TM at 17°C. The highest progression obtained after the 72 h was 34% as observed from the semen kept at 17°C in Biladyl and 45% in the semen kept at 17°C Triladyl. With suitable protocol, both extenders can maintain goat sperm motility at specific refrigerated temperatures when kept fresh for up to 72 h.

Key Words: Boer buck, ambient, artificial vaginal