A study was conducted to assess blood profiles of Ross 308 broiler and indigenous Venda chickens. A total of 120 Ross 308 broiler and Venda chickens were raised for a period of 90 d on a commercial grower diet containing 20% crude protein and 13 MJ ME/kg DM. Ten chickens per breed were randomly selected at 42 and 90 d of age and 3 mL of blood samples from both chicken breeds were collected from the jugular vein into 2 test tubes per bird to determine the blood profiles. A t-test was used to test the significance of differences between breeds (P < 0.05). Differences among hematological parameters of Ross-308 and Venda chickens at 42 d of age were not significant (P > 0.05) except for platelets ( × 10³µ/L) for Ross 308 broiler (59.2 ± 10.13) and Venda chickens (24.0 ± 10.00); serum creatinine (µmol/L) for Ross 308 broiler (17.5 ± 50.05) and Venda chickens (9.0 ± 5.02) and total protein (g/L) of Venda (25.5 ± 3.20) and Ross 308 broiler chickens (20.0 ± 3.12). At 90 d of age, white blood cells (734.4 ± 18.97 × 10³ cell/µL), red blood cells (2.3 ± 0.15; ×10⁶ cell/µL), mean corpuscular volume (121.1 ± 4.10 f/L), serum calcium (2.6 ± 0.01 mmol/L), creatinine (10.7 ± 0.76 µmol/L), cholesterol (3.9 ± 0.12 mmol/L) and albumin (9.5 ± 0.23 g/L) were higher for Ross 308 broiler chickens. However, platelets (×10³µ/L) were higher in Venda (14.4 ± 3.29) than in Ross 308 broiler (12.2 ± 3.00) chickens. In the adult stage, broiler chickens have better hematological properties than indigenous chickens. Further investigation on immunological parameters of these breeds needs to be conducted.