To investigate the effects of a blend of organic acids and medium chain fatty acids on gut health, 96 weaned barrows (21 d; BW 5.2 kg) were housed (3 pigs/pen) with ad libitum access to feed and water over a 28-d study. The diets were comprised primarily of corn, soybean meal and a source of lactose, with inclusion of 0, 0.15, 0.30 and 0.45% of a mixture of acetic acid, citric acid, formic acid, lactic acid, caprylic acid and caproic acid (BLEND). Piglets (n = 4 pigs/treatment/time point) were euthanized on d 14 and 28 for sample collection. Two-cm segments of duodenal and jejunal tissue were collected for analysis of apoptotic and mitotic rates. Rate of apoptosis and mitosis were evaluated immunohistochemically in paraffin wax-embedded tissue sections using polyclonal antibodies (anti-Caspase 3 and anti-PCNA, respectively; Biocare Medical, Concord, CA).The immunohistochemical procedure was conducted per the manufacturer’s instructions. Images were recorded using an Olympus BX41 camera. For each intestinal sample, a minimum of 20 to 30 full villi and crypts were measured. ANOVA was generated using the mixed-model procedure of SAS. Linear, quadratic, and cubic polynomials were used to detect the response to dietary inclusion of the BLEND. All measurements were performed without treatment awareness. A cubic effect was observed with respect to the number of cells in mitosis in duodenal (P < 0.0001) and jejunal (P = 0.0062) tissue segments obtained on d 14 with the greatest rate of mitosis observed in tissues obtained from pigs fed 0.30% BLEND. In addition, a quadratic effect in mitosis:apoptosis (P = 0.0171) ratio was observed in duodenal tissue obtained from piglets on d 14 with minimal inflection point at 0.18% inclusion of BLEND. No significant effects were observed with respect to mitotic or apoptotic rates in tissues obtained on d 28. In conclusion, feeding a blend of organic acids and medium chain fatty acids may have a short-term positive effect on mitosis and apoptosis, possibly contributing to intestinal epithelial restitution and gut health.