Abstract #M365

# M365
Development of an in vitro subacute ruminal acidosis (SARA) model.
Allan B. Chestnut*1, Jim M. Aldrich1, Tammy K. Miller Webster2, Wenping Hu1, Wibe B. Fokkink1, Howard G. Bateman1, 1Provimi North America, Brookville, OH, 2Rumen Fermentation Profiling Laboratory, West Virginia University, Morgantown, WV.

To test efficacy of additives to reduce SARA, a protocol was developed to model SARA conditions using an in vitro continuous culture fermentor system at the Rumen Fermentation Profiling Laboratory, West Virginia University. A BASE diet was formulated with a 60:40 forage:concentrate (F:C) ratio (DM basis). Inclusion of corn silage, alfalfa balage, grass hay, ground corn (GC), SBM, corn gluten meal, corn gluten feed, soybean hulls, dried molasses, sodium bicarbonate and a mineral-vitamin supplement were, respectively, 36.1, 12.1, 12.1, 11.00, 13.7, 1.0, 1.0, 8.3, 2.0, 2.0 and 1.8% of DM. Mean particle size of GC was 870 µm (SD = 2.7). In Trial 1, diet SARA1 was formulated by reducing the BASE diet F:C ratio to 52:48. Corn was increased to 20.9% with 50% (DM basis) of the GC replaced with steam flaked corn (SFC). Treatments were: BASE diet fed as 25-g meals 4 times daily for 10 d (B) and BASE diet fed as 25-g meals 4 times daily for 7 d followed by the SARA1 diet fed as 50-g meals 2 times daily for 3 d (S1). Treatments were replicated in triplicate. Data collected the last 3 d included pH at 30-min intervals and NDF digestibility. Daily average pH were calculated for each fermentor. All data were analyzed using PROC MIXED of SAS. Average and minimum pH were analyzed with a repeated-measures model. Fermentor was treated as a random variable and AR(1) was selected as the appropriate covariance structure. Average pH of S1 (6.25) vs B (6.28) was similar (P = 0.15). Minimum pH of S1 (6.06) vs B (6.15) tended to be less (P = 0.08). NDF digestion was less (P < 0.01) for S1 (41.5%) vs B (46.6%). In Trial 2, treatment S1 was replaced with S2 by altering the SARA1 diet to create SARA2 as follows: corn replaced sodium bicarbonate and the GC:SFC ratio changed to 1:2. Trial 2 protocol was similar to Trial 1. Average pH of S2 (6.17) was less (P < 0.05) than B (6.30). Minimum pH of S2 (5.88) was less (P < 0.01) than B (6.18). NDF digestion was less (P < 0.01) for S2 (30.5%) vs B (40.4%). It was concluded that using the tested protocol with diets BASE and SARA2 provided an appropriate SARA model.

Key Words: subacute ruminal acidosis, in vitro, model