Objectives of this study were to evaluate the effects of live yeast (LY; Saccharomyces cerevisiae boulardii strain I-1079; 0.35 × 10⁹ cfu/g ProTernative) supplementation during the receiving period on growth efficiency, feeding behavior, activity and vaginal temperature in 72 newly weaned beef heifers (initial BW of 203 ± 22 kg). Immediately upon weaning, heifers were vaccinated (Pyramid 5) and ship stressed (800 km) before being returning to the research center. Upon arrival, heifers were allotted to 1 of 4 pens each equipped with 3 GrowSafe feed bunks, and pens to 1 of 2 treatments (n = 36) consisting of standard receiving diet (ME 2.36 Mcal/kg, CP 16.5% DM) without LY, and control diet containing LY (5 g ProTernative/kg diet; Lallemand Animal Nutrition). Temperature sensors (iButton) were placed intra-vaginally (CIDR) to record temperature, and HOBO devices attached (hind leg) to measure physical activity for the first 14 d (n = 18). LY treatment did not affect morbidity rate (10.4%), vaginal temperature (39.2 ± 0.2°C), or frequency (16.6 ± 2.2 events/d) and duration (46 ± 5 min/event) of standing bouts. ADG tended (P < 0.1) to be greater for LY heifers during the first 28 d (0.625 vs. 0.432 ± 0.08 kg/d), but was not affected by LY treatment during the 56-d study. LY treatment did not affect DMI, but DMI increased as the study progressed from 2.06 ± 0.22 (first 14 d) to 2.91 ± 0.19% of BW during the 56-d study. LY heifers consumed more (P < 0.05) meals (16.8 vs. 14.6 vs. ± 1.1 events/d) that were shorter (P = 0.08) in length (12.8 vs 14.9 ± 1.2 min/event) and smaller (P < 0.05) in size (0.48 vs 0.55 ± 0.04 kg/event) and at a slower (P < 0.05) meal-eating rate (4.61 vs. 5.54 ± 0.39 g DM/min) compared with control heifers. Moreover, heterogeneities of DMI (SD = 0.59 vs. 0.92 kg/d) and RFI (SD = 0.48 vs. 0.73 kg/d) were less (P < 0.05) in LY than control heifers. While the LY treatment did not affect growth efficiency or health status, supplementation with live-yeast may have favorably affected meal patterns of newly weaned beef heifers.