Abstract #M347

# M347
Energy expenditure and methane emission in dairy heifers using the face-mask method.
Carlos Alberto Alves Oliveira Filho1, Fernanda Samarini Machado2, Alexandre Lima Ferreira2, Luiz Gustavo Ribeiro Pereira*2, Thierry Ribeiro Tomich2, Mariana Magalhães Campos2, José Augusto Gomes Azevêdo3, Rogério Martins Maurício4, Alexandre Vieira Chaves5, Camilla Flávia Portela Gomes Silva6, 1Universidade Estadual do Sudoeste da Bahia, Itapetinga, Bahia, Brazil, 2Embrapa Dairy Cattle, Juiz de Fora, Minas Gerais, Brazil, 3Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil, 4Universidade Federal de São João Del Rei, São João Del Rei, Minas Gerais, Brazil, 55Faculty of Veterinary Science, Sydney, New South Wales, Australia, 66Instituto Federal de Educação, Ciência e Tecnologia Baiano, Santa Inês, Bahia, Brazil.

The aim of this study was to evaluate the effect of feeding levels (FL) and breed (B) on energy expenditure as heat production (HP) and methane emission in dairy heifers using the face-mask method. Twenty-four heifers, 8 Holstein, 8 Gyr and 8 Holstein-Gyr crossbreed (F1) with average live weight (LW) of 440 ± 88 kg and average age of 27.5 ± 0.8 mo, were housed in tie stall and randomly distributed to the treatments in a 2 × 3 factorial design (feeding level of 1.17% LW or 1.46% LW, on dry matter (DM) basis and breed). The diet was offered as a total mixed ration (700g/kg of corn silage and 300g/kg of concentrate, on DM basis). O2 consumption, CO2 production and CH4 emission data were measured using Sable System (Sable Systems, Henderson, NV) coupled with a face-mask for 30 min per day, for 3 d. Heart rate (HR) (beat/min) was registered over 72 h using Polar Equine transmitter (model RS800CX G3, Polar Electro Enc.). Three measurements of oxygen pulses (O2P) (mL O2/beat) were registered. Total daily O2 consumption (L/d) was calculated as O2P times daily mean HR. Daily HP was calculated as total daily O2 consumption times the constant 20.47 KJ/L of O2. Data were subjected to ANOVA and means were compared by Student-Newman-Keuls test (P < 0.05). No effect of the interaction between B and FL was observed for any of the variables analyzed. HR, O2P and HP (kcal/kg LW0.75) did not differ among B and FL. F1 heifers presented higher DM intake (DMI) and higher daily mean gain (DMG) in comparison to Holstein and Gyr breeds (6.18, 5.36 and 4.14 kg DM/d; 674.3, 480.8 and 435.4 g/d, respectively). Animals fed at 1.46% LW level presented higher DMI, DMG, BW and methane emission (g/d). Methane emission was higher for F1 animals (161.3 g/d), but did not differ from Holstein breed (141.1 g/d). Animals from Gyr breed fed at 1.17% LW presented lower DMI, DMG and BW as well as inferior CH4 production (98.3 g/d). This research project was funded by FAPEMIG, CAPES, CNPq and Embrapa.

Key Words: greenhouse gas, heat production, zebu