Abstract #M211

# M211
Reproductive performance of lactating dairy cows with an extended duration of the postpartum voluntary waiting period and injectable trace mineral supplementation.
Matias L. Stangaferro*1, Robert Wijma1, Magdalena Masello1, Rodrigo C. Bicalho2, Mark J. Thomas3, Julio O. Giordano1, 1Department of Animal Science, Cornell University, Ithaca, NY, 2Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, NY, 3Dairy Health and Management Services, Lowville, NY.

Objectives were to evaluate (1) the effect of extending the duration of the voluntary waiting period (VWP) from 60 to 88 DIM; and (2) the effect of injectable trace mineral (Zn, Mn, Se, Cu) supplementation (TMS) on reproductive performance of dairy cows. Holstein cows [n = 1,105; 441 primiparous (PP) and 664 multiparous (MP)] were blocked by parity and total milk production in their previous lactation (MP only) and assigned to a 2x2 treatment arrangment to receive: TMS or NoTMS and first timed-AI (TAI) at 60 ± 3 (SVWP) or 88 ± 3 DIM (LVWP) after synchronization of ovulation with the Double-Ovsynch protocol (GnRH-7d-PGF-3d-GnRH-7d- GnRH-7d-PGF-56h-GnRH-16h-TAI) resulting in the following groups: TMS-SVWP (n = 315), NoTMS-SVWP (n = 325), TMS-LVWP (n = 228) and NoTMS-LVWP (n = 237). Cows in TMS received 3 SQ injections of 5 mL of TMS (Multimin 90®) as follows: 2 injections prepartum at 229 ± 3 and 259 ± 3 d of gestation for MP and 243 ± 3 and 263 ± 3 d of gestation for PP. A third injection was given at 20 ± 3 d before first TAI. The effect of VWP duration, TMS, parity, and their interaction on pregnancy per AI (P/AI) at 39 d after AI, pregnancy loss (PL), and cows pregnant by 90 DIM (PG90) were evaluated by logistical regression. At 39 d after AI, P/AI was greater for cows in the LVWP (47.5%) than SVWP (39.1%) group. At 39 d after AI, TMS did not affect (P = 0.96) P/AI (42.7% and 42.5% for TMS and NoTMS). Primiparous cows had greater (P < 0.01) P/AI than multiparous cows (49.0% and 38.4%). Pregnancy losses were similar (P = 0.68) for cows in LVWP (5.8%) and SVWP (4.7%) and for cows in TMS (5.7%) and NoTMS (4.9%). Also, PL were similar (P = 0.13) for PP (3.1%) and MP (7.1%) cows. No differences (P = 0.59) in PG90 were observed for cows in LWVP (46.2%) and SVWP (48.0%) and for cows in the TMS (48.3%) and NoTMS (46.0%) group (P = 0.49). Conversely PG90 was greater (P < 0.01) for PP (52.5%) than MP (43.7%) cows. We conclude that extending the duration of the VWP from 60 to 88 DIM improved P/AI of lactating dairy cows. Conversely, TMS supplementation during the prepartum period and 20 d before TAI did not improve fertility of lactating dairy cows.

Key Words: voluntary waiting period, trace mineral, dairy cow