Abstract #840

# 840
Evaluation of Ovsynch and CIDR inserted concurrently with or two days after initiation of Ovsynch protocol to improve reproductive performance in lactating dairy cows with low estrus detection efficiency.
Abid Hussain Shahzad*1, Abdul Sattar1, Nasim Ahmad1, Ijaz Ahmad1, Deniz Nak2, Yavuz Nak2, 1University of Veterinary and Animal Sciences, Lahore, Lahore, Punjab, Pakistan, 2Uludag University, Bursa, Bursa, Turkey.

To assess the efficiency of 3 synchronization methods as postpartum reproductive management tools in dairy cattle on a commercial dairy farm. Cyclic Holstein lactating cows (n = 167),55–90 DIM, were randomly allotted to 1 of 3 treatments. First group was (Ovsynch: n = 58) given a 2-mL i.m. injection of synthetic GnRH on d 0. After 7 d, PGF2a (2 mL i.m.) was injected. A second injection of GnRH was administered 48 h after the PGF2a injection and all cows were bred by timed AI 16 h after the second GnRH injection. Cows in second group (Ovsynch+P7: n = 55) were introduced a modified Ovsynch TAI treatment similar to Ovsynch but CIDR was inserted for a 7-d period (CIDR was inserted with GnRH and removed on PG Shot). In third group (Ovsynch+P5; n = 54), cows were subjected to Ovsynch protocol in addition to CIDR insertion for 5 d (CIDR was inserted after 2 d of GnRH and removed with PG shot). P4 conc. was measured on d 30 and 60. Pregnancy diagnosis was carried out at d 30, 60, 90 post insemination in all 3 groups. Progesterone profile was significantly higher (P < 0.05) for Ovsynch + P5 (7.75 ± 0.38 ng/mL) and Ovsynch + P7 (7.58 ± 0.26 ng/mL) compared with Ovsynch (6.52 ± 0.32 ng/ mL) on d 30 PTAI but nonsignificant among all 3 synchronization treatments (i.e., 6.37 ± 0.49 in Ovsynch, 6.75 ± 0.36 in Ovsynch+P5 and 6.80 ± 0.41 in Ovsynch+P7 group). Pregnancy rate in Control group was 39.7, 36.2, and 32.80 on d 30, 60 and 90 post insemination. In second modified Ovsynch group with 7d CIDR (Ovsynch+P7) insertion pregnancy rate was 42.6% on d 30, 37% on d 60 and 90 PAI. In third group pregnancy rate was 45.5, 43.6 and 41.8 on corresponding 30, 60, 90 d PAI. Although pregnancy rate were statistically not significant but, by percentage points, improved pregnancy rate was observed on all 3 time points in Ovsynch+p5 group.

Key Words: modified Ovsynch, postpartum lactating cow, progesterone